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KMID : 0355219960210020331
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Journal of Korean Academy Oral Medicine
1996 Volume.21 No. 2 p.331 ~ p.350
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Immunological Study of Induction to Salivary Glands The Cytochrome P450(IIEl) by Stress in Rat
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Abstract
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Cytochronre P450 is an oxidase involved in oxidation of alcohol and is known to be an activator of carcinogen.
The present study was performed to study the effect of alcohol and cold stress on the expression of Cytochrome P450 IIE1(CYPIIEI) in the liver and salivary glands in rats by an immunoblot analysis.
Sixteen rats were divided into 4 groups; 1)rats belonging to group I were allowed to take 15%(v/v) ethyl alcohol as a drink ad libitum; 2)rats of group II were bathed in cold water for 30 sec twice a day (during the one-week experiment); 3)rats comprising group III were received alcohol and cold stress as described above; 4)rats of group IV were selected as a control.
The rats were sacrificed at the.end of the one-week experimet. The livers and parotid and submandibular salivary glands were removed. and stored at -20°C until: use. The stored organs were homogenized for 10 sec and -the supernatants were obtained by centrifugation. The proteins of the supernatants were separated by sodium . dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to Western blotting. The blotted membranes were incubated with polyclonal antibodies to CYPIIEI.
The obtained results were as follows
l. The expression of CYPIIE1 was apparently negative in the liver and salivary glands of group IV, wheras its expression was marked in the experiment groups I, II, and III.
2. No difference in the expression of CYPIIE1 in the liver and salivary glands was observed between the experiment groups I, II, and III.
3. Among the experiment groups, the expression of CYPIIE1 in the liver was much greater than in the salivary glands. The expression of CYPIIEI in the submadibular gland was weakly positive but was greater than in the parotid gland.
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